The G-CSF, now known as Colony Stimulating Factor 3 (CSF3) is produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Many cell types (fibroblasts, astrocytes, endothelial cells, and bone marrow stromal cells) secreted CSF3 after LPS, IL-1 or TNFα activation. CSF3 is known to regulate the production, differentiation, and function of granulocytes and the monocytes/macrophages. It may also function in some adhesion or recognition events at the cell surface.

 

CSF3 cDNA (Genbank accession number XM_001095097.4) codes for a 200 aa polypeptide precursor including a 26 aa signal peptide (resulting in a 174 aa mature polypeptide) that is 95.48 % homologous to human CSF3.

 

rMamu CSF3 is produced as a mature polypeptide in HEK293 cells with TEV protease digestion site to remove the extraneous residues at the aminoterminus.

 

Purity is verified by PAGE electrophoresis and absence of endotoxin by LAL test.

 

The activity of rMamu CSF3 remains to be determined using M-NFS-60 cells.