FLT3L acts as both a cytokine and a growth factor, acting as a multipotent progenitor factor, inducing the production of immune cells, but also the generation and maturation of dendritic cells, both plasmacytoid and myeloid DCs.
rMamuFLT3L cDNA codes for a 236 aa precursor protein with a 26 aa leader sequence amounting to a 210 aa mature protein that is 95.3 % homologous to human FLT3L.
rMamuFLT3L is produced as a soluble protein in E.coli using the pET32 expression vector with an enterokinase digestion site to ensure the absence of extraneous residues at the amino terminus.
Purity is verified by PAGE electrophoresis and absence of endotoxin by LAL test.
Bioactivity of each lot is tested based on its ability to induce proliferation of primary mouse bone marrow cells in vitro.