DR5 is a ligand for TRAIL and can be used to block TRAIL in vivo and in vitro.
 
rMamuDR5 is 445 aa polyprotein, (5 aa more than human DR5) that shares only 88.2% amino acid sequence identity with the human homologue. The signal peptide is 53 aa, the removal of which yields a mature protein consisting of 392 aa.
 
rMamuDR5 is produced by the Resource as a soluble DR5-IgG1-Fc (LALA mutant) to block TRAIL in vivo or in vitro and produced in  Pichia pastoris followed by purification with Protein G sepharose. Alternatively soluble DR5-His6 can be produced in E-coli and purified over a Nickel column.
 
Purity is verified by PAGE electrophoresis and absence of endotoxin by LAL test.

 Rhesus DR5

 

 1  MGQLRQSAPA ASGARKGRGP GPREARGARP GLRVLKTLVL VVAAAAVLLS VSADCAPITR   61 QSLDPQRRAA PQQKRSSPIE GLCPPGHHIS EDSRECISCK YGQDYSTHWN DFLFCLRCTK 121 CDSGEVEVNS CTTTRNTVCQ CEEGTFREED SPEICRKCRT GCPRGMVKVK DCTPWSDIEC 181 VHKESGTKHT GEVPAVEKTV TTSPGTPASP CSLSGIIIGV IVLVVIVVVA VIVWKTSLWK 241 KVLPYLKGVC SGGGGDPERV DSSSHSPQRP GAEDNALNEI VSIVQPSQVP EQEMEVQEPA 301 EQTDVNTLSP GESEHLLEPA KAEGPQRRGQ LVPVNENDPT ETLRQCFDDF AAIVPFDAWE 361 PLVRQLGLTN NEIKVAKAEA ASSRDTLYVM LIKWVNKTGR AASVNTLLDA LETLEERLAK 421 QKIQDRLLSS GKFMYLEDNA DSATS

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Alignment of human and rhesus macaque DR5 amino acid sequence.

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Amino acid differences in bold (18.2%), signal peptide underlined.